跳转至内容
Merck
CN
  • Two glycine riboswitches activate the glycine cleavage system essential for glycine detoxification in Streptomyces griseus.

Two glycine riboswitches activate the glycine cleavage system essential for glycine detoxification in Streptomyces griseus.

Journal of bacteriology (2014-01-21)
Takeaki Tezuka, Yasuo Ohnishi
摘要

The glycine cleavage (GCV) system catalyzes the oxidative cleavage of glycine into CO2, NH4(+), and a methylene group, which is accepted by tetrahydrofolate (THF) to form N(5),N(10)-methylene-THF. Streptomyces griseus contains gcvP and the gcvT-gcvH operon, which encode three intrinsic components of the GCV system. We identified the transcriptional start sites of gcvTH and gcvP and found putative glycine riboswitches in their 5' untranslated regions (5' UTRs). The ratios of the transcripts of the gcvT and gcvP coding sequences (CDSs) to those of the respective 5' UTRs were significantly higher in the presence of glycine in the wild-type strain. However, the levels of gcvT and gcvP CDS transcripts were not increased by glycine in the respective 5' UTR deletion mutants. A reporter gene assay showed that a transcriptional terminator exists in the 5' UTR of gcvTH. Furthermore, by an in-line probing assay, we confirmed that glycine bound directly to the putative riboswitch RNAs. These results indicate that the S. griseus glycine riboswitches enhance transcriptional read-through to the downstream CDSs, like known glycine riboswitches in other bacteria. We examined the growth of three mutants in which either or both of the gcvTH and gcvP 5' UTRs were deleted. Like the wild-type strain, all mutants grew vigorously in a medium containing 0.9% glucose as a carbon source. However, the mutants showed severely restricted growth in a medium containing 0.9% glucose and 1% glycine, while the wild-type strain grew normally. This indicates that glycine has a growth-inhibitory effect and that the GCV system plays a critical role in glycine detoxification in S. griseus.

材料
Product Number
品牌
产品描述

Sigma-Aldrich
甘氨酸, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
甘氨酸, suitable for electrophoresis, ≥99%
Sigma-Aldrich
甘氨酸, BioUltra, Molecular Biology, ≥99.0% (NT)
Sigma-Aldrich
甘氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, ≥98.5%
Sigma-Aldrich
甘氨酸 盐酸盐, ≥99% (HPLC)
SAFC
甘氨酸
Supelco
甘氨酸, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
甘氨酸, BioXtra, ≥99% (titration)
Sigma-Aldrich
甘氨酸, ACS reagent, ≥98.5%
Sigma-Aldrich
甘氨酸, 99%, FCC
Sigma-Aldrich
甘氨酸, meets analytical specification of Ph. Eur., BP, USP, 99-101% (based on anhydrous substance)
USP
甘氨酸, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
甘氨酸, puriss. p.a., reag. Ph. Eur., buffer substance, 99.7-101% (calc. to the dried substance)
Sigma-Aldrich
1 M 甘氨酸 溶液
Supelco
甘氨酸, analytical standard, for nitrogen determination according to Kjeldahl method
甘氨酸, European Pharmacopoeia (EP) Reference Standard
Supelco
甘氨酸 盐酸盐 溶液, 100 mM amino acid in 0.1 M HCl, analytical standard
Supelco
甘氨酸, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
甘氨酸, tested according to Ph. Eur.
Sigma-Aldrich
甘氨酸, Vetec, reagent grade, 98%