CN

Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol.

Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol.

FEBS letters (2015-04-12)

Suguru Komenoi, Fumika Takemura, Hiromichi Sakai, Fumio Sakane

摘要

Diacylglycerol kinase (DGK) η plays important roles in various patho-physiological events such as oncogenesis. In this study, we performed an enzymological characterization of DGKη splice variant 1 (DGKη1). The Km value for diacylglycerol was 0.14 mol%. Intriguingly, the Km value of DGKη1 for diacylglycerol was at least 9-fold lower than those of other DGK isozymes including DGKα, indicating that DGKη1 is a high affinity isozyme for diacylglycerol. Therefore, DGKη1 is a unique DGK isozyme, which may function at particular membrane sites where only low concentrations of diacylglycerol are supplied.

材料

货号

品牌

产品描述

Sigma-Aldrich

HEPES, ≥99.5% (titration)

Sigma-Aldrich

氯化钠, BioXtra, ≥99.5% (AT)

Sigma-Aldrich

HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

氯化钠, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Sigma-Aldrich

DL-二硫代苏糖醇溶液, BioUltra, for molecular biology, ~1 M in H₂O

Sigma-Aldrich

氯化镁溶液, for molecular biology, 1.00 M±0.01 M

Sigma-Aldrich

氯化钠溶液, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

氯化镁, anhydrous, ≥98%

Sigma-Aldrich

氯化钠溶液, 0.9% in water, BioXtra, suitable for cell culture

Sigma-Aldrich

氯化钠溶液, 5 M

Supelco

DL-二硫代苏糖醇溶液, 1 M in H₂O

Sigma-Aldrich

氯化钠, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

Sigma-Aldrich

氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

SAFC

氯化钠溶液, 5 M

Sigma-Aldrich

HEPES缓冲溶液, 1 M in H₂O

Sigma-Aldrich

HEPES, BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

氯化镁溶液, BioUltra, for molecular biology, ~1 M in H₂O

Sigma-Aldrich

氯化钠溶液, BioUltra, for molecular biology, ~5 M in H₂O

SAFC

HEPES

Sigma-Aldrich

氯化钠, 99.999% trace metals basis

Sigma-Aldrich

氯化钠, BioUltra, for molecular biology, ≥99.5% (AT)

Sigma-Aldrich

氯化镁, powder, <200 μm

SAFC

HEPES

Sigma-Aldrich

氯化镁溶液, BioUltra, for molecular biology, 2 M in H₂O

Sigma-Aldrich

HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

氯化钠溶液, 0.85%

Sigma-Aldrich

氯化镁, BioReagent, suitable for insect cell culture, ≥97.0%

Sigma-Aldrich

氯化镁溶液, PCR Reagent, 25 mM MgCI₂solution for PCR

Sigma-Aldrich

氯化钠, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

Sigma-Aldrich

HEPES, BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)