CN

Steady-state cross-correlations for live two-colour super-resolution localization data sets.

Steady-state cross-correlations for live two-colour super-resolution localization data sets.

Nature communications (2015-06-13)

Matthew B Stone, Sarah L Veatch

摘要

Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.

材料

货号

品牌

产品描述

Sigma-Aldrich

HEPES, ≥99.5% (titration)

Sigma-Aldrich

氯化钠, BioXtra, ≥99.5% (AT)

Sigma-Aldrich

HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

甲醛溶液, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

Sigma-Aldrich

L -还原型谷胱甘肽, ≥98.0%

Sigma-Aldrich

氯化钠, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Sigma-Aldrich

氯化镁溶液, for molecular biology, 1.00 M±0.01 M

Sigma-Aldrich

甲醛溶液, for molecular biology, 36.5-38% in H₂O

Sigma-Aldrich

氯化钙溶液, BioUltra, for molecular biology, ~1 M in H₂O

Sigma-Aldrich

氯化镁, anhydrous, ≥98%

Sigma-Aldrich

氯化钠溶液, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

氯化钠溶液, 0.9% in water, BioXtra, suitable for cell culture

Sigma-Aldrich

N-羟基丁二酰亚胺, 98%

Sigma-Aldrich

氯化钠溶液, 5 M

Sigma-Aldrich

氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

氯化钠, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

SAFC

氯化钠溶液, 5 M

Sigma-Aldrich

L -还原型谷胱甘肽, suitable for cell culture, BioReagent, ≥98.0%, powder

Sigma-Aldrich

氯化钙, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

HEPES缓冲溶液, 1 M in H₂O

Sigma-Aldrich

氯化镁溶液, BioUltra, for molecular biology, ~1 M in H₂O

Sigma-Aldrich

甲醛溶液, for molecular biology, BioReagent, ≥36.0% in H₂O (T)

Sigma-Aldrich

HEPES, BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

己酸, ≥99%

Sigma-Aldrich

氯化钠溶液, BioUltra, for molecular biology, ~5 M in H₂O

Sigma-Aldrich

氯化镁, powder, <200 μm

Sigma-Aldrich

氯化钠, BioUltra, for molecular biology, ≥99.5% (AT)

Sigma-Aldrich

氯化镁溶液, BioUltra, for molecular biology, 2 M in H₂O

Sigma-Aldrich

氯化钙, anhydrous, powder, 99.99% trace metals basis

SAFC

HEPES