Merck
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HPLC Analysis of Diet Soda Additives on Ascentis® Express C18

HPLC Analysis of Diet Soda Additives on Ascentis® Express C18 application for HPLC

材料

分析色谱柱

产品编号
说明
价格

Ascentis® Express C18, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 3 cm × 4.6 mm

标准

产品编号
说明
价格

咖啡因

Sigma Reference Standard, vial of 250 mg

山梨酸

99.0-101.0% anhydrous basis

安赛蜜

阿斯巴甜

analytical standard

SS苯甲酸

2000 μg/mL in dichloromethane, analytical standard

纽甜

analytical standard

CONDITIONS

column

Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)

mobile phase

20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min

pressure

3191 psi (220 bar)

flow rate

3 mL/min

column temp.

40 °C

detector

UV, 214 nm

injection

1 μL

sample

(Diet Soda 100 - 500 μg/mL in buffer)

说明

分析说明

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet cola.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany