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HPLC Analysis of Hae III Digest of pBR22 DNA on TSKgel® NPR, High Resolution, Fast Analysis
材料
保护柱
产品编号
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价格
TSKgel® DNA-NPR Guard Column
phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 5 μm particle size分析色谱柱
产品编号
说明
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TSKgel® DEAE-NPR HPLC Column
phase DEAE (diethylaminoethyl), L × I.D. 3.5 cm × 4.6 mm, 2.5 μm particle size流动相组分
产品编号
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CONDITIONS
column
TSKgel DEAE-NPR, 3.5 cm x 4.6 mm I.D., 2.5 μm particles (813075)
column
TSKgel DEAE-NPR gaurd column, 0.5 cm x 4.6 mm I.D., 5 μm particles (817088)
mobile phase
[A] 0.02 M Tris-HCl, pH 9.0; [B] 0.02 M Tris-HCl, 1.0 M NaCl, pH 9.0
gradient
48 to 65% B in 15 min
flow rate
1.5 mL/min
pressure
2000 psi 138 bar
column temp.
40 °C
detector
UV, 260 nm
sample
Hae III Digest of pBR22 DNA, base pair number for each peak is indicated on the figure
说明
其他说明
Data provided by Tosoh Bioscience LLC.
法律信息
TSKgel is a registered trademark of Tosoh Corporation