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HPLC Analysis of Proteins on BIOshell™ A400 Protein C4, Rapid Separation at Elevated Temperature

HPLC Analysis of Proteins on BIOshell™ A400 Protein C4, Rapid Separation at Elevated Temperature application for HPLC

材料

used together

产品编号
说明
价格

66830-U

BIOshell™ A400 Protein C4, 3.4 μm Guard Cartridge

3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea

66841-U

Guard Cartridge Holder for BIOshell™ Guard Column

相关产品

产品编号
说明
价格

G8511

β-Galactosidase from Escherichia coli

For use as a marker in SDS-PAGE

L1254

L-Lactic Dehydrogenase from rabbit muscle

Type XI, lyophilized powder, 600-1,200 units/mg protein

L6876

Lysozyme from chicken egg white

powder or granules, ≥39,000 units/mg protein

H0138

Haptoglobin Human, Phenotype 1-1

98-100%, essentially salt-free, lyophilized powder

66824-U

BIOshell™ A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 5 cm × 2.1 mm

CONDITIONS

column

BIOshell A400 Protein C4, 5 cm x 2.1 mm I.D., 3.4 μm particles (66824-U)

mobile phase

[A] 75:25 (0.1% TFA in water):(0.1% TFA in acetonitrile); [B] 25:75 (0.1% TFA in water):(0.1% TFA in acetonitrile)

gradient

12 to 100% B in 1 min; held at 100% B for 1 min

flow rate

0.4 mL/min

pressure

905 psi (62 bar)

column temp.

90 °C

detector

UV, 215 nm

injection

1 μL

sample

protein mix in 0.05% TFA

说明

分析说明

This application demonstrates the suitability of BIOshell A400 Protein C4 column for fast elution of proteins via ballistic gradients. High temperature enhances chromatographic performance and lowers operational backpressure. The highest grade solvents provided clean, robust operation.

法律信息

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS