HPLC Analysis of Proteins on Proteomix® SCX-NP5
材料
used together
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说明
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产品编号
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Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell cultureRibonuclease A from bovine pancreas
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg proteinProteomix® SCX-NP5 Strong Cation Exchange Column
L × I.D. 5 cm × 4.6 mm, 5 μm particle size, stainless steelCONDITIONS
column
Proteomix SCX-NP5, 5 cm x 4.6 mm I.D., 5 μm particles (Z777159)
mobile phase
[A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient
0 to 60% in 20 min
flow rate
0.3 mL/min
pressure
400 psi total pressure (28 bar)
column temp.
25 °C
detector
UV, 250 nm
injection
5 μL
sample
ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
说明
分析说明
The separation of four readily investigated proteins was examined on a Proteomix SCX-NP5 column in order to examine retention time and resolution. The four peaks were well resolved in under 15 minutes.
法律信息
Proteomix is a registered trademark of Sepax Technologies