Proteomix SCX-NP5, 5 cm x 4.6 mm I.D., 5 μm particles (Z777159)
[A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
0 to 60% in 20 min
0.3 mL/min
400 psi total pressure (28 bar)
25 °C
UV, 250 nm
5 μL
ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
The separation of four readily investigated proteins was examined on a Proteomix SCX-NP5 column in order to examine retention time and resolution. The four peaks were well resolved in under 15 minutes.
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