TSKgel BioAssist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)
[A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
0 to 60% in 20 min, 60 to 100% in 0.5 min, held for 5 minutes
0.3 mL/min
400 psi total pressure (28 bar)
25 °C
UV, 280 nm
5 μL
ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
Four commonly investigated proteins were chromatographed on a TSKgel BioAssist S cation exchange column. This column was found to provide better resolution than the Proteomix WCX-NP5, but less resolution between the proteins than the Proteomics SCX-NP5.
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