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HPLC Analysis of Proteins on TSKgel® BioAssist S

HPLC Analysis of Proteins on TSKgel® BioAssist S application for HPLC

材料

used together

产品编号
说明
价格

452289

磷酸

≥85 wt. % in H2O, ≥99.999% trace metals basis

相关产品

产品编号
说明
价格

C4879

α-糜蛋白酶原A 来源于牛胰腺

essentially salt-free, lyophilized powder

L6876

Lysozyme 来源于鸡蛋白

powder or granules, ≥39,000 units/mg protein

C3131

线粒体蛋白 来源于牛心脏

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

R4875

核糖核酸酶A 来源于牛胰腺

Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

819686

TSKgel® BioAssist® S HPLC Column

phase sulfonic acid, L × I.D. 5 cm × 4.6 mm, 7 μm particle size, PEEK hardware

CONDITIONS

column

TSKgel BioAssist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)

mobile phase

[A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid

gradient

0 to 60% in 20 min, 60 to 100% in 0.5 min, held for 5 minutes

flow rate

0.3 mL/min

pressure

400 psi total pressure (28 bar)

column temp.

25 °C

detector

UV, 280 nm

injection

5 μL

sample

ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L

说明

分析说明

Four commonly investigated proteins were chromatographed on a TSKgel BioAssist S cation exchange column. This column was found to provide better resolution than the Proteomix WCX-NP5, but less resolution between the proteins than the Proteomics SCX-NP5.

法律信息

TSKgel is a registered trademark of Tosoh Corporation

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS