HPLC Analysis of Reduced SILu™ Lite SigmaMAb on Zenix® SEC-300 with pH-Neutral Buffer
CONDITIONS
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
mobile phase
[A] 0.2 M sodium phosphate dibasic, pH 7.0 with phosphoric acid; [B] water; [C] acetonitrile; (50:20:30, A:B:C)
flow rate
0.3 mL/min
pressure
1070 psi (74 bar)
column temp.
25 °C
detector
UV, 215 nm
injection
1 μL
sample
reduced SigmaMAb (with 10 mM dithiobutylamine), 1 g/L, in mobile phase A
说明
分析说明
The integrity of antibodies in the development of biologics is of the utmost importance in the pharmaceutical quality control lab; therefore, a method to examine intact antibodies versus reduced antibody subunits is needed. Separation of the light and heavy chains of the SigmaMAb antibody standard, was achieved using size exclusion chromatography on a Zenix SEC-300 column.
法律信息
SILu is a trademark of Sigma-Aldrich Co. LLC
Zenix is a registered trademark of Sepax Technologies