HPLC Analysis of Sulfa Drugs on Titan C18

Titan C18 5 cm x 2.1 mm I.D., 1.9 μm particle (577122-U)

[A] water with 0.1% acetic acid; [B] acetonitrile with 0.1% acetic acid ; (90:10, A:B)

0.4 mL/min

2670 psi (184 bar)

40 °C

UV, 250 nm

2 μL

25 μg/mL in 70:30, water:methanol

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