HPLC Analysis of Sunscreens on Ascentis^® C18

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] water, [B] methanol, (88:12, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 305 nm

injection

10 μL

sample

50 μg/mL in mobile phase