HPLC Analysis of Warfarin Enantiomers on Astec^® CHIROBIOTIC^® V (Method 2)

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

[A] 0.1% triethylamine acetate, pH 5.0; [B] THF; (80:20, A:B)

1 mL/min

ambient

UV, 254 nm

1 μL

4 mg/mL in methanol

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany, CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, null