Ascentis Express F5, 5 cm x 3.0 mm I.D., 2.7 μm particles (53576-U)
[A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile
0% B for 1.5 min, 0-10% over 0.5 min; to 15% B in 1.5 min, 2 min re-equilibration at 0% B
1.5 mL/min
4931 psi (340 bar)
35 °C
UV, 210 nm
5 μL
all vitamins in water, 10 μg/mL for nicotinic acid and folic acid, 12 μg/mL for thiamine hydrochloride and cyanocobalamin, (20 μg/mL for pyridoxine, 30 μg/mL for riboflavin, 40 μg/mL for niacineamide, 100 μg/mL for calcium pantothenate, 120 μg/mL for biotin)
A mix of 9 water-soluble B-vitamins was separated on Ascentis Express F5 column
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany