LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18
材料
分析色谱柱
标准
Ascomycin solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Cyclosporin D solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
mobile phase
[A] 0.1% formic acid water; [B] 0.1% formic acid in 98:2 (v/v) acetonitrile:water
gradient
50% B for 2 min; to 100% B in 2 min; held for 2 min
flow rate
0.4 mL/min
column temp.
65 °C
detector
MS, ESI(+), MRM
sample
300 ng/mL in (4:1) 1% formic acid acetonitrile:water
injection
5 μL
column
apHera C18, 15 cm x 2.1 mm I.D., 5 μm particles (56100AST)
说明
分析说明
The apHera C18 HPLC column packed with polymeric particles demonstrated excellent peak shape and rapid elution for these bulky, immunosuppressant compounds under conditions not permitted by silica-based HPLC columns. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
法律信息
apHera is a trademark of Sigma-Aldrich Co. LLC