Sample spiked into rat plasma
HybridSPE®-PLus 96-Well Plate (575659-U)
100 uL of spiked rat plasma was added to the HybridSPE plate well, followed by 300 uL of protein precipitation solvent, acetonitrile with 1% formic acid. (The plate, sealed, secured on a vibrater, agitated by vibration at 1000 rpm for 2 min. The plate was transfered to a vacuum manifold. Vacuum was applied at 10 in Hg for 4 min.)
Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)
[A] Water; [B] methanol, both with 0.1% formic acid/10 mM ammonium formate
0-1 min, 45%B; 1.5-4 min 90%B, 4.5-8 min 45%B
0.2 mL/min
2900 psi
35 °C
MS, ESI(+), MRM mode
1 μL
Sample pretreatment: Sample spiked into rat plasma
Detector:
MRM transitions:
Peak MRM
1. Quinidine325.2/81.1, 307.1
2. Verapamil455.3/165.1
3. Digoxin798.5/651.5
4. Amiodarone646.1/58.1, 100.1
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