LC/MS/MS Analysis of Steroids on Ascentis® Express C18
材料
相关产品
产品编号
说明
价格
11-脱氧皮质激素标准液 溶液
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®21-脱氧皮质激素标准液 溶液
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®脱氢表雄酮（DHEA）标准液 溶液
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®5α-二氢睾酮(DHT)标准液 溶液
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®17α-Methyltestosterone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] 0.1% formic acid in water ; [B] methanol
gradient
5%B to 70%B in 3 minutes, then to 90%B in 0.1 minute, hold 90%B for 0.9 minutes
flow rate
0.5 mL/min
pressure
4351 psi (300 bar)
column temp.
40 °C
detector
MS/MS, ESI(+), MRM (See Figure for transitions)
injection
10 μL
sample
10 ng/mL in methanol
说明
分析说明
Fast and selective method for steroids.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany