liquid infant formula
SupelMIP Bisphenol A, 100 mg/3 mL (52775-U)
3 mL 2% formic acid in methanol, 3 mL acetonitrile, 3 mL water
as prepared
9 mL of water, 6 mL of acetonitrile:water (40:60); vacuum was pulled for 3 min after the last wash
Sample was evaporated to dryness at 45 °C, reconstuituted in 0.5 mL of acetonitrile:water (40:60). All samples were filtered through Durapore membrane filter units prior to analysis.
Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
[A] water; [B] acetonitrile
40 to 80% B in 2.5 min; held for 4.5 min; to 100%B in 0.1 min; held for 2.4 min; to 40% B in 0.1 min; held for 2.9 min
0.25 mL/min for 7 min; to 0.40 mL/min in 0.1 min; held for 2.4 min; to 0.25 mL/min in 0.1 min.; held for 2.9 min
3916 psi (270 bar)
35 °C
fluorescence, ex: 230 nm, em: 315 nm
5 μL
3 mL of methanol:acetonitrile (75:25) (A017)
BPA can leach into foods from the contact between food and the packaging. Structurally analogous compounds could also be used in place of BPA during package manufacturing as BPA comes under more stringent regulations. The presented method was developed for analysis of this class of compounds and applied to infant formula analysis. The use of molecularly imprinted polymers during cleanup produced samples that can be analysed by fluoresent detection.
Sample Prep: 5 mL of liquid infant formula was spiked with 10 ppb of analytes, mixed with 5.5 mL of acetonitrile, centrifuged at 4500 rpm for 10 minutes, top 3 mL were diluted with 3 mL water to load into SPE
SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC
Titan is a trademark of Sigma-Aldrich Co. LLC