inhibitors. J. Med. Chem., 46, 1-4 (2003).
3. Blong, R.M., et al., Tetramerization domain of
human butyrylcholinesterase is at the C-terminus.
Biochem. J., 327, 747-57 (1997).
4. Supplier Data
5. Masson
inhibitors. J. Med. Chem., 46, 1-4 (2003).
4. Blong, R.M., et al., Tetramerization domain of
human butyrylcholinesterase is at the C-terminus.
Biochem. J., 327, 747-57 (1997).
5. Lee, J.C., and Harpst
inhibitors. J. Med. Chem., 46, 1-4 (2003).
3. Blong, R.M., et al., Tetramerization domain of
human butyrylcholinesterase is at the C-terminus.
Biochem. J., 327, 747-57 (1997).
4. Lee, J.C., and Harpst
trifluoroethanol.
3. Add 20 L of the denaturing solution to the dried
sample.
4. Incubate at 57 C for 1 hour.
5. Alkylate the reduced sample by adding 5 L of
200 mM iodoacetamide
trifluoroethanol.
3. Add 20 L of the denaturing solution to the dried
sample.
4. Incubate at 57 C for 1 hour.
5. Alkylate the reduced sample by adding 5 L of
200 mM iodoacetamide
trifluoroethanol.
3. Add 20 L of the denaturing solution to the dried
sample.
4. Incubate at 57 C for 1 hour.
5. Alkylate the reduced sample by adding 5 L of
200 mM iodoacetamide
trifluoroethanol.
3. Add 20 L of the denaturing solution to the dried
sample.
4. Incubate at 57 C for 1 hour.
5. Alkylate the reduced sample by adding 5 L of
200 mM iodoacetamide
yeast is a
tetramer consisting of subunits of approximately
57 kDa. The molecular weights of its monomer, dimer,
trimer, and tetramer are 57 kDa, 114 kDa, 171 kDa,
and 228 kDa, respectively. The monomers
stomacher.
2. Incubate at 37 °C ± 1 °C for 23 – 25 h.
3. After incubation, mix and inoculate 0.1 mL of culture in 10
mL full Fraser broth.
4. Incubate at 37 °C ± 1 °C for 22 – 26 h.
5.
days at 37 °C.
References
1. Massoulie, J., et al., Progress in Neurobiology, 41,
31-91 (1993).
2. Jbilo, O., et al., Toxicon, 32, 1445-1457 (1994).
3. Ott, P., et al., Eur. J. Biochem., 57, 469-
: E2005
(2018).
8. Taniguchi K., et al., Mol Carcinog., 57, 579-589
(2018).
9. Abdelhaleem M., Biochim Biophys Acta., 1704,
37-46 (2004).
Notice
We provide information and advice to our
sample at 37 °C for
10-20 seconds in a water bath, then transfer the
sample to the cuvet preheated to 37 °C.
References
1. Luduena, R.F., et al., Current Opinion in Cell
Biology, 4
the product at –20 C.
References
1. Horinouchi, S. et al., Appl. Environ. Microbiol.,
57(5), 1386-1393 (1991).
TRITON is a trademark of The Dow Chemical Company
or an affiliated company
Avoid repeated freezing and
thawing.
Procedure
4-Nitrophenol Hydroxylase Activity
A 0.5 ml reaction containing the following was
incubated at 37 °C for 30 minutes:
50 pmoles of cytochrome P450 isozyme
inhibitor of xanthine
oxidase-catalyzed production of superoxide
radical. Biochem. Pharmacol., 37(2), 349-352
(1988).
4. Stryer, L., Biochemistry, 3rd ed., W.H. Freeman
(New York, NY: 1988), pp. 619-621.