CN
qPCR
应用筛选条件
facet applications:qPCR
facet content type:Technical Article
显示 31-47 共 47 条结果
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Quantitative RT-PCR
RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
RT-PCR / RT-qPCR Troubleshooting
Developing a PCR or RT-PCR/RT-qPCR troubleshooting protocol so that data are reliable is essential. Potential sources of RT-PCR or PCR error and problems include operator error, the PCR master mix, and oligo design. This PCR troubleshooting guide outlines and details
Direct and Crude Extract qPCR
Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE.
MIQE – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Our qPCR services, including primer and probe designs, assay protocol development, troubleshooting, and data analysis support, adhere to MIQE, which allows you to publish or bring your product to market faster and with confidence.
Quantitative PCR Basics
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Dual-Labeled Probes
Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
RNA Immunoprecipitation (RIP) FAQs
RNA Immunoprecipitation (RIP) Frequently Asked Questions
KAPA SYBR® FAST qPCR Kits FAQs
Review some of the most asked questions regarding the KAPA SYBR FAST qPCR kits, which provide optimal performance under strict real-time PCR reaction conditions, and improve the signal-to-noise ratio (fluorescence), quantitation cycle (Cq), linearity and sensitivity.
KiCqStart® SYBR® Green Primers FAQs
Review some of the most asked questions regarding KiCqStart® Primers, which are pre-designed primer pairs for quantifying gene expression by SYBR® Green I RT-qPCR (reverse-transcription quantitative real-time PCR).
Locked Nucleic Acid
Frequently asked questions about Locked Nucleic Acids (LNA)
How qPCR Works
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
PCR/qPCR/dPCR Assay Design
PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.
2/2