qPCR

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Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher

Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.

Water for Next-Generation Sequencing

Explore the essential role of water in next-generation sequencing for accurate and efficient genomic analysis

Dual-Labeled Probes

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

RT-PCR Troubleshooting

PCR troubleshooting guide for reliable data in molecular assays.

Quantitative PCR Basics

Quantitative PCR detection builds on basic PCR techniques and allows researchers to estimate the quantity of starting material in a sample with a much wider dynamic range.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Troubleshooting PCR and RT-PCR Amplification

This page shows PCR and RT-PCR amplification troubleshooting.

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

Primer and Probe Design

Video: Primer and Probe Design

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Complete Solutions for PCR Assay Development

Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.

Custom Oligos Modifications

We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.

PCR Technologies Guide

Basic PCR/qPCR/dPCR protocols for assay quality control and specific studies.

Array CGH Analysis of Challenging Samples

aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

Molecular Beacons

Learn about precision Sigma-Aldrich Molecular Beacon oligonucleotide probes used for sequence detection in qPCR for in vitro research.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Choosing the Right Custom qPCR Probe

View a summary of the applications using commonly ordered custom qPCR probes.

DNA Oligonucleotide Synthesis

Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

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