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Merck
CN
  • Staphylococcus aureus cell wall stress stimulon gene-lacZ fusion strains: potential for use in screening for cell wall-active antimicrobials.

Staphylococcus aureus cell wall stress stimulon gene-lacZ fusion strains: potential for use in screening for cell wall-active antimicrobials.

Antimicrobial agents and chemotherapy (2008-06-11)
Rebecca Steidl, Stacy Pearson, Robert E Stephenson, Nagender Ledala, Sutthirat Sitthisak, Brian J Wilkinson, Radheshyam K Jayaswal
摘要

lacZ fusion strains were constructed using the promoters of five cell wall stress stimulon genes: pbp2, tcaA, vraSR, sgtB, and lytR. All fusion strains were induced only in the presence of cell wall-active antibiotics, suggesting the potential of these strains for use in high-throughput screening for new cell wall-active agents.

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