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Assessment of bacterial resistance to organic solvents using a combined confocal laser scanning and atomic force microscopy (CLSM/AFM).

Assessment of bacterial resistance to organic solvents using a combined confocal laser scanning and atomic force microscopy (CLSM/AFM).

Journal of microbiological methods (2014-09-07)

Maria S Kuyukina, Irena B Ivshina, Irina O Korshunova, Ekaterina V Rubtsova

摘要

Using combined confocal laser scanning and atomic force microscopy (CLSM/AFM), bacterial viability under organic solvent stress was assessed at single cell level. Solvent-exposed bacteria stained with the LIVE/DEAD BacLight fluoresced green or red, allowing viable and dead cell discrimination. However, with toluene, butanol and acetonitrile, dually fluorescent cells appeared having compromised cell membranes. Changes in size, surface/volume ratio and roughness were revealed as possible resistance mechanisms.

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