跳转至内容

CN

The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.

The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.

Steroids (2015-03-12)

Peter J Crick, Lien Beckers, Myriam Baes, Paul P Van Veldhoven, Yuqin Wang, William J Griffiths

摘要

Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5pg/mL-2.6ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma.

材料

产品编号

品牌

产品描述

Sigma-Aldrich

2-丙醇, BioReagent, ≥99.5%, Molecular Biology

Sigma-Aldrich

乙腈, anhydrous, 99.8%

Sigma-Aldrich

纯乙醇, 200 proof, anhydrous, ≥99.5%

Sigma-Aldrich

胆固醇, Sigma Grade, ≥99%

Sigma-Aldrich

甲醇, anhydrous, 99.8%

Sigma-Aldrich

磷酸二氢钾一元, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

Sigma-Aldrich

2-丙醇, anhydrous, 99.5%

Sigma-Aldrich

纯乙醇, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

异丙醇, ≥99.7%, FCC, FG

Sigma-Aldrich

胆固醇, powder, BioReagent, suitable for cell culture, ≥99%

Sigma-Aldrich

磷酸二氢钾一元, ReagentPlus^®

Sigma-Aldrich

2-丙醇, BioUltra, Molecular Biology, ≥99.5% (GC)

Sigma-Aldrich

磷酸二氢钾一元, Molecular Biology, ≥98.0%

Sigma-Aldrich

纯乙醇, 190 proof, meets USP testing specifications

Sigma-Aldrich

SyntheChol ^® NS0 补充, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture

Sigma-Aldrich

乙酸, suitable for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

胆固醇, from sheep wool, ≥92.5% (GC), powder

Sigma-Aldrich

2-丙醇, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

乙腈, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

磷酸二氢钾一元, BioUltra, Molecular Biology, anhydrous, ≥99.5% (T)

Sigma-Aldrich

甲酸, ≥95%, FCC, FG

Sigma-Aldrich

乙酸, ≥99.5%, FCC, FG

Sigma-Aldrich

乙酸, natural, ≥99.5%, FG

Supelco

10% (v/v) 乙醇标准品, 10 % (v/v) in H₂O, analytical standard

Sigma-Aldrich

磷酸二氢钾一元, 99.99% trace metals basis

Sigma-Aldrich

80% v/v 乙醇固定液, suitable for fixing solution (blood films)

Sigma-Aldrich

甲醇, suitable for NMR (reference standard)

SAFC

胆固醇, from sheep wool, Controlled origin, meets USP/NF testing specifications

Sigma-Aldrich

甲醇溶液, suitable for NMR (reference standard), 4% in methanol-d₄ (99.8 atom % D), NMR tube size 3 mm × 8 in.

Sigma-Aldrich

纯乙醇, 190 proof, ACS reagent, meets USP testing specifications, Excise Tax-free, Permit for use required