LC/MS Analysis of Conjugated Bile Acids on Ascentis® Express C18
材料
used together
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CONDITIONS
column
Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)
mobile phase
[A] 10 mM ammonium acetate, pH 4 with acetic acid; [B] acetonitrile
gradient
25 to 40% B in 10 min
flow rate
1.0 mL/min (postcolumn split to MS)
column temp.
35 °C
detector
MS, ESI(-), SIR
injection
5 μL
sample
10 mg/L each in mobile phase A
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany