LC-MS Anlaysis of Pain Managment Opiates on Ascentis® Express Biphenyl, 30 x 2.1 mm
材料
相关产品
产品编号
说明
价格
13种疼痛管理混标 溶液
100 μg/mL each component (10 μg/mL Fentanyl), ampule of 1.0 mL, certified reference material, Cerilliant®CIS 盐酸曲马多标准液 盐酸盐 溶液
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Biphenyl, 3 cm x 2.1 mm, 2.7 μm particles (64054-U)
mobile phase
[A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient
10 to 20% B in 0.75 min; to 95% B in 1.5 min
flow rate
0.3 mL/min
pressure
2125 psi (147 bar)
column temp.
30 °C
detector
MS-TOF, ESI+
injection
2 μL
sample
50-500 ng/mL in 99:1, water:methanol
说明
分析说明
This application demonstrates the separation of 13 pain managment opiates on the Ascentis Express Biphenyl using an LC-TOF mass spectrometer. This application demonstrates the scalability of this method to 30 x 2.1 mm column geometry using the 2.7 um biphenyl phase. The method provides baseline resolution of all isobaric sets of interest.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany