UHPLC-MS Analysis of Basic Drugs and Metabolites with Titan C18

Titan C18 10 cm x 2.1 mm, 1.9 μm particles (577124-U)

[A] Water; [B] acetonitrile, both with 0.1% formic acid

35%B Isocratic

0.4 mL/min

484 psi (7120)

25 °C

MS, ESI(+), MRM

1 μL

50 ng/mL in 50% methanol

MRM transitions
1. Quinidine325.2/81.2
2. Propranolol260.1/116.2
3. Haloperidol376.1/165.1
4. Haloperidol metabolite II378.1/149.1
5. Haloperidol cholor analog392.1/181.0
6. Fluoxetine 310.1/44.2

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