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UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion

UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion application for UHPLC

材料

used together

产品编号
说明
价格

575662-U

Supel™ — Select HLB 固相萃取 96 孔板

bed wt. 60 mg/well, pk of 1

G2174

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata)

aqueous solution, ≥85,000 units/mL

相关产品

产品编号
说明
价格

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奥沙西泮标准液 溶液

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

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1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-905

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1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

A-902

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100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

A-903

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1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

A-911

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A-916

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1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

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100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

A-925

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100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

D-907

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1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

F-003

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Flunitrazepam-d7

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L-021

Lorazepam glucuronide solution

100 μg/mL in acetonitrile: water (1:1), ampule of 1 mL, certified reference material, Cerilliant®

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1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

SPE well plate

Supel-Select HLB 96-well plate, 60 mg/well (575662-U)

condition

1 mL acetonitrile, 1 mL water

sample addition

1.1 mL enzyme-digested urine

washing

1 mL water, 1 mL 25% methanol

elution

1 mL acetonitrile

column

Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)

mobile phase

[A]: 5 mM ammonium formate [B]: 5 mM ammmonium formate in acetonitrile:water (95:5)

gradient

10 to 90% B in 2.5 min, held for 0.5 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+), MRM

injection

2 μL

说明

分析说明

Urine samples were spiked with various glucuronide metabolites of benzodiazepines along with the parent drugs.  The glucuronide metabolites were converted back to the parent drug using β-glucuronidase, then processed using SPE prior to LC/MS analysis. The Supel-Select HLB provided clean extracts and the Titan C18 provided rapid resolution of parent drugs and metabolites. Cerilliant CRMs provided reliable quantitation.

法律信息

Supel is a trademark of Sigma-Aldrich Co. LLC
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