size+exclusion+chromatography

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Efficient Bioseparation Technique: Size exclusion chromatography (SEC) effectively separates molecules by size, a crucial method in commercial processes.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Column Packing and Preparation for Size Exclusion Chromatography

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Analytical Size-exclusion Chromatography Columns for Biomolecule Separations

Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.

Workflow and Analysis of Stressed and Unstressed Antibody Samples By Size Exclusion Chromatography

A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.

Columns and Analytical Standards for Protein SEC

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

Size Exclusion Analysis of Tween-Containing IgG Formulations

In this study, we investigated the effect of adding Tween® 20 and Tween 80 on the elution of proprietary mAb 221 under size exclusion chromatography conditions.

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.

Capto Core 700

Capto Core 700 from Cytiva is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.

Afﬁnity Chromatography in a Puriﬁcation Strategy (CIPP)

This page shows the puriﬁcation strategy of capture, intermediate puriﬁcation, and polishing (CIPP) in affinity chromatography using Cytiva products.

Mesoporous Materials: Properties and Applications

Mesoporous Materials include a range of high surface area porous silicates with applications in gas adsorption, drug delivery, diagnostics and catalysis.

Biopolymer Separations

Supelco’s product offering for biopolymer separations includes columns and media categorized by separation mode, as well as by column brand.

HPLC Analysis of PEG g006200

HPLC Analysis of PEG on Two Zenix® SEC-300 Columns in Tandem

HPLC Columns for Antibody and Large Protein Analysis

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

Protein Concentration & Buffer Exchange

Overview of techniques used to concentrate and clarify protein samples for purification, bioprocessing, and analysis workflows. Includes protocols and videos for filtration and ultrafiltration techniques, protein enrichment, and desalting and buffer exchange using dialysis, diafiltration, and chromatography methods.

Protein Research

A comprehensive offering of protein preparation products cell and tissue extraction, high-yield isolation and purification, and application-verified detection tools for molecular biology and drug discovery.

Protein Conformation Array Multiplexing

Monoclonal antibodies (mAbs) are the fastest growing class of human therapeutics within the field of biologics, having predicted worldwide sales of $125 billion by 2020 based on current approval rates.

Duolink® Proximity Ligation Assay

Duolink® Proximity Ligation Assay (PLA) technology for detecting and quantifying protein-protein interactions.

Electron Transport

Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.

Dextran and Related Polysaccharides

Learn about C6H10O6 or dextran formation, classes and naming. Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, which have a linear backbone of α-linked d-glucopyranosyl repeating units.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Egg-based and Cell-based Influenza Vaccine Manufacturing Methods

Influenza vaccines are commonly made using egg-based and cell-based manufacturing strategies. Find step-by-step information on the manufacturing process for each method.

Laboratory Dialysis & Diafiltration Devices

Dialyzers, centrifugal ultrafiltration devices, and stirred cells for buffer exchange, dialysis, desalting, and diafiltration of 10 µL – 1,200 mL sample volumes.

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

Nucleases (DNases and RNases)

Nucleases, including high-purity DNase, RNase, and phosphodiesterase enzymes to support your nucleic acid digestion application needs.

Whole Cell Lysis Reagents & Enhancers

Protein extraction kits, cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and mammalian cell cultures and tissue samples.

Targeted Protein Degradation

Targeted protein degradation (TPD) is an emerging drug discovery strategy that uses small-molecules, such as proteolysis-targeting chimeras (PROTACs), to eradicate targeted proteins linked to disease from cells.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Detergents REACH Information

Explore our comprehensive portfolio of detergents and surfactants as possible Triton X-100 alternatives for your research applications before the REACH Annex 14 ban takes effect.

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