产品名称
Anti-P-Glycoprotein Mouse mAb (C494), liquid, clone C494, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
C494, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, hamster
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
Positive Control
Cell lines or frozen tissue, such as human liver or colon.
Cell lines or frozen tissue, such as human liver or colon.
General description
This Anti-P-Glycoprotein Mouse mAb (C494) is validated for use in Paraffin Sections, FC, Frozen Sections, Immunoblotting, ICC, IP for the detection of P-Glycoprotein.
Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein.
Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein. Does not cross-react with the MDR3 P-glycoprotein.
Application
Paraffin Sections (5-10 µg/ml, see comments)
Flow Cytometry (see comments)
Frozen Sections (5-10 µg/ml, see comments)
Immunoblotting (see comments)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Flow Cytometry (see comments)
Frozen Sections (5-10 µg/ml, see comments)
Immunoblotting (see comments)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Disclaimer
Toxicity: Standard Handling (A)
Immunogen
Epitope: gene-specific internal cellular epitope present only on the MDR1 isoform of P-glycoprotein
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a human leukemic cell line
Other Notes
Does not cross-react with MDR3 P-glycoprotein. Also reported to work for flow cytometry, immunoblotting, and immunoprecipitation. For immunocytochemistry, frozen or paraffin-embedded material may be used. Permeabilization of the cell membrane is required to expose the epitope recognized by this antibody. Variables associated with assay conditions will dictate the proper working dilutions.
Rao, V.V., et al. 1994. Cancer Res.54, 1536.
Toth, K., et al. 1992. Am. J. Pathol.140, 1009.
Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 152.
Toth, K., et al. 1992. Am. J. Pathol.140, 1009.
Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 152.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1% BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regino Mercado-Lubo et al.
Nature communications, 7, 12225-12225 (2016-07-28)
Salmonella enterica serotype Typhimurium is a food-borne pathogen that also selectively grows in tumours and functionally decreases P-glycoprotein (P-gp), a multidrug resistance transporter. Here we report that the Salmonella type III secretion effector, SipA, is responsible for P-gp modulation through
Amélie Petitprez et al.
International journal of oncology, 42(5), 1644-1653 (2013-04-03)
Irinotecan is a major anticancer agent specifically targeting DNA topoisomerase I. Its cytotoxicity is mediated via a two-step process involving accumulation of reversible DNA‑topoisomerase I complexes associated with transient DNA single-strand breaks which subsequently are converted into permanent DNA double-strand
Golam Kibria et al.
Biological & pharmaceutical bulletin, 37(12), 1926-1935 (2014-12-03)
Multi-drug resistance (MDR) of cancers to chemotherapy including doxorubicin (DOX) is mediated by several factors. To design an effective therapy for the treatment of chemotherapy-resistant cancers, it is essential to explore the elements responsible for mediating MDR. However, exploring these
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