跳转至内容
Merck
CN
  • A new quantitative method to determine the uptake of SPIONs in animal tissue and its application to determine the quantity of nanoparticles in the liver and lung of Balb-c mice exposed to the SPIONs.

A new quantitative method to determine the uptake of SPIONs in animal tissue and its application to determine the quantity of nanoparticles in the liver and lung of Balb-c mice exposed to the SPIONs.

Journal of biomedical nanotechnology (2013-05-01)
R D Zysler, E Lima, M Vasquez Mansilla, H E Troiani, M L Mojica Pisciotti, P Gurman, A Lamagna, L Colombo
PMID23627077
摘要

We propose a new method for determining the quantity of superparamagnetic iron oxide nanoparticles (Fe3O4, SPIONs) embedded in animal tissue using magnetization measurements. With this method, the smallest detectable quantity of magnetite nanoparticles in a tissue sample is -1 microg. We showed that this method has proved being efficient. In this study, we focused in determining the quantity of SPION confined in lung and liver tissue of mice injected with -13 nm magnetite superparamagnetic nanoparticles. Furthermore, the method allowed us to detect the magnetite nanoparticles present in animal tissues without letting the natural iron ions present in the tissue or blood interfere with the measurements.

材料
Product Number
品牌
产品描述
31390
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr ~70,000
31389
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr ~40,000
31392
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr 450,000-650,000
31388
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr ~6,000
D5376
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 1,500,000-2,800,000
D1662
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 35,000-45,000
D9260
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 9,000-11,000
D8821
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 60,000-76,000
09184
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr ~100,000
00271
Supelco
右旋糖酐, analytical standard, 25,000
D4876
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 150,000
31425
Supelco
葡聚糖 来源于肠系膜明串珠菌, analytical standard, suitable for gel permeation chromatography (GPC), Mw 670,000
00269
Supelco
右旋糖酐, analytical standard, 5,000
31430
Supelco
右旋糖酐, analytical standard, suitable for gel permeation chromatography (GPC), Set Mp 1,000-400,000
00896
Supelco
右旋糖酐, analytical standard, 670,000
00893
Supelco
右旋糖酐, analytical standard, 150,000
00894
Supelco
右旋糖酐, analytical standard, 270,000
00268
Supelco
右旋糖酐, analytical standard, 1,000
31398
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, Mr ~200,000
00270
Supelco
右旋糖酐, analytical standard, 12,000
31387
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr 15,000-25,000
D8802
Sigma-Aldrich
葡聚糖 溶液 来源于肠系膜明串珠菌, 20 % (w/w) (Autoclaved)
31397
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, Mr ~60,000
00895
Supelco
右旋糖酐, analytical standard, 410,000
31394
Sigma-Aldrich
右旋糖酐, enzymatic synth.
00891
Supelco
右旋糖酐, analytical standard, 50,000
31417
Supelco
葡聚糖 来源于肠系膜明串珠菌, analytical standard, suitable for gel permeation chromatography (GPC), Mw 5,000
00892
Supelco
右旋糖酐, analytical standard, 80,000
31416
Supelco
葡聚糖 来源于肠系膜明串珠菌, analytical standard, Mw 1,000
D3759
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 48,000-90,000