HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision
材料
相关产品
产品编号
说明
价格
Superoxide Dismutase from bovine erythrocytes
BioReagent, ≥3,000 units/mg protein, suitable for cell culture, lyophilized powderGlucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)CONDITIONS
column
BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)
mobile phase
[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
gradient
10 to 100% B in 18 min
flow rate
0.3 mL/min
pressure
2800 psi total pressure (193 bar)
column temp.
30 °C
detector
UV, 215 nm
injection
5 μL
sample
superoxide dismutase, 0.13; a-lactalbumin, 0.13; serum albumin bovine, 0.13; β-lactoglobulin A, 0.06; catalase, 0.13; (carbonic anhydrase, 0.13; β-amylase, 0.13; glucose-6 phosphate dehydrogenase, 0.13 g/L in mobile phase A)
说明
分析说明
Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
法律信息
BIOshell is a trademark of Sigma-Aldrich Co. LLC