BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)
[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
20 to 100% B in 8 min
0.3 mL/min
2480 psi total pressure (193 bar)
30 °C
UV, 215 nm
2 μL
lysozyme, 0.06; haptoglobulin, 0.06; conalbumin, 0.12; tryspin inhibitor, 0.18; glutamate dehydrogenase, (0.18; ß-galactosidase, 0.12; serum albumin chicken, 0.12 g/L in mobile phase A)
Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
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