[Double bonds of fatty acids, alcohols glycerol, cholesterol and nonpolar serum lipids. Diagnostic value of hypercholesterolemia].

A method for evaluation of double bonds (DB) by ozone titration in a pool of serum lipids during extraction after Folch is developed. A method for measuring the weight of nonpolar lipids in apoB-100-lipoproteins (LP) with a hydrophobic fluorescent probe K-37 was used. Cholesterol and glycerol alcohols were measured in the serum using Cholesterol and Triglycerides kits (Hoffmann la Roche). Twenty-four patients with myocardial infarction, 29 coronary patients, and 37 healthy children were examined. The level of cholesterol alcohol reflects the number of double bonds in fatty acids (FA) and hence, the content of essential polythenic FA in the serum. The higher the level of cholesterol alcohols the greater the pool of essential poly-FA, circulating in the blood as nonpolar cholesterol polyesters within low density lipoproteins (LDL). Presumably, the higher the level of cholesterol LDL, the more pronounced is the deficit of essential poly-FA and the greater is the compensatory activation of the synthesis of endogenous poly-FA. A positive correlation between the content of cholesterol alcohol in the serum and weight of nonpolar lipids in apoB-100-LP was noted.