Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 μL of a 100 ppm solution of nonane (internal standard)
100 μm polydimethylsiloxane (57300-U)
headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm
250 °C for 5 min
SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
40 °C, 3 °C/min to 250 °C (2 min)
250 °C
FID, 260 °C (sampling rate 80 ms)
helium, 35.0 cm/sec
splitless
0.75 mm I.D. SPME (2633901)
Limoncello liquor
In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany