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HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2) application for HPLC

材料

标准

产品编号
说明
价格

C4011

细胞色素 C 来源于鸽子胸肌

≥95% based on Mol. Wt. 12,173 basis

C4879

α-糜蛋白酶原A 来源于牛胰腺

essentially salt-free, lyophilized powder

C2037

线粒体蛋白 来源于牛心脏

≥95% based on Mol. Wt. 12,327 basis

C3131

线粒体蛋白 来源于牛心脏

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

细胞色素 C 来源于马心脏

suitable for GFC marker, BioReagent

C7752

细胞色素 C 来源于马心脏

≥95% based on Mol. Wt. 12,384 basis

C2506

细胞色素 C 来源于马心脏

≥95% (SDS-PAGE)

C2436

细胞色素 C 来源于酿酒酵母

≥85% based on Mol. Wt. 12,588 basis

R5125

核糖核酸酶A 来源于牛胰腺

Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R5000

核糖核酸酶A 来源于牛胰腺

Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R6513

核糖核酸酶A 来源于牛胰腺

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

核糖核酸酶A 来源于牛胰腺

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

R5500

核糖核酸酶A 来源于牛胰腺

Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

R4875

核糖核酸酶A 来源于牛胰腺

Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R5250

核糖核酸酶A 来源于牛胰腺

Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5503

核糖核酸酶A 来源于牛胰腺

Type I-AS, 50-100 Kunitz units/mg protein

C4129

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

C3483

线粒体蛋白 来源于人类心脏

≥95% (SDS-PAGE), lyophilized powder

30398

细胞色素 C 来源于牛心脏

≥95% (GE)

C2867

细胞色素 C 来源于马心脏

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

CONDITIONS

column

Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)

mobile phase

[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)

flow rate

0.5 mL/min

injection

10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

detector

UV, 280 nm

说明

分析说明

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

法律信息

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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