Merck
CN
  • The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

Genome research (2004-10-19)
Daniela S Gerhard, Lukas Wagner, Elise A Feingold, Carolyn M Shenmen, Lynette H Grouse, Greg Schuler, Steven L Klein, Susan Old, Rebekah Rasooly, Peter Good, Mark Guyer, Allison M Peck, Jeffery G Derge, David Lipman, Francis S Collins, Wonhee Jang, Steven Sherry, Mike Feolo, Leonie Misquitta, Eduardo Lee, Kirill Rotmistrovsky, Susan F Greenhut, Carl F Schaefer, Kenneth Buetow, Tom I Bonner, David Haussler, Jim Kent, Mark Kiekhaus, Terry Furey, Michael Brent, Christa Prange, Kirsten Schreiber, Nicole Shapiro, Narayan K Bhat, Ralph F Hopkins, Florence Hsie, Tom Driscoll, M Bento Soares, Tom L Casavant, Todd E Scheetz, Michael J Brown-stein, Ted B Usdin, Shiraki Toshiyuki, Piero Carninci, Yulan Piao, Dawood B Dudekula, Minoru S H Ko, Koichi Kawakami, Yutaka Suzuki, Sumio Sugano, C E Gruber, M R Smith, Blake Simmons, Troy Moore, Richard Waterman, Stephen L Johnson, Yijun Ruan, Chia Lin Wei, S Mathavan, Preethi H Gunaratne, Jiaqian Wu, Angela M Garcia, Stephen W Hulyk, Edwin Fuh, Ye Yuan, Anna Sneed, Carla Kowis, Anne Hodgson, Donna M Muzny, John McPherson, Richard A Gibbs, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha Madan, Stephanie Rodrigues, Amy Sanchez, Michelle Whiting, Anup Madari, Alice C Young, Keith D Wetherby, Steven J Granite, Peggy N Kwong, Charles P Brinkley, Russell L Pearson, Gerard G Bouffard, Robert W Blakesly, Eric D Green, Mark C Dickson, Alex C Rodriguez, Jane Grimwood, Jeremy Schmutz, Richard M Myers, Yaron S N Butterfield, Malachi Griffith, Obi L Griffith, Martin I Krzywinski, Nancy Liao, Ryan Morin, Ryan Morrin, Diana Palmquist, Anca S Petrescu, Ursula Skalska, Duane E Smailus, Jeff M Stott, Angelique Schnerch, Jacqueline E Schein, Steven J M Jones, Robert A Holt, Agnes Baross, Marco A Marra, Sandra Clifton, Kathryn A Makowski, Stephanie Bosak, Joel Malek
ABSTRACT

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nucleoside Phosphorylase bacterial, recombinant, expressed in E. coli, ≥10 units/mg protein
Sigma-Aldrich
Phosphomannose Isomerase from Escherichia coli, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein
Sigma-Aldrich
Tyrosinase from mushroom, lyophilized powder, ≥1000 unit/mg solid
Sigma-Aldrich
Dihydrofolate Reductase human, ≥80% (SDS-PAGE), recombinant, expressed in E. coli, ≥1 units/mg protein
Sigma-Aldrich
Galactokinase human, recombinant, expressed in E. coli
Sigma-Aldrich
Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 units/mg protein
Sigma-Aldrich
Lipase from Mucor miehei, lyophilized powder, ≥4,000 units/mg solid (using olive oil)
Sigma-Aldrich
β-Galactosidase from Escherichia coli, aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Sigma-Aldrich
Glutaminase from Escherichia coli, Grade VII, lyophilized powder, 500-1,500 units/mg protein
Sigma-Aldrich
Adenosine 5′-Triphosphatase from porcine cerebral cortex, lyophilized powder, ≥0.3 units/mg protein, pH 7.8
Sigma-Aldrich
Pyruvate Carboxylase from bovine liver, buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)
Sigma-Aldrich
β-Galactosidase from bovine testes, ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)
Sigma-Aldrich
Pyruvate Kinase from rabbit muscle, Type III, lyophilized powder, 350-600 units/mg protein
Sigma-Aldrich
L-Amino Acid Oxidase from Crotalus adamanteus, Type I (dried venom)
Sigma-Aldrich
Lipase from Candida rugosa, Type VII, ≥700 unit/mg solid
Sigma-Aldrich
Lipase from Candida rugosa, lyophilized powder, ≥40,000 units/mg protein
Sigma-Aldrich
Aconitase from porcine heart
Sigma-Aldrich
Enolase from baker's yeast (S. cerevisiae), lyophilized powder, ≥50 units/mg protein
Supelco
GAPDH, standard for protein electrophoresis
Sigma-Aldrich
Pyruvate Kinase from rabbit muscle, Type VII, buffered aqueous glycerol solution, 350-600 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein
Sigma-Aldrich
Creatine Phosphokinase from rabbit muscle, Type I, salt-free, lyophilized powder, ≥150 units/mg protein
Sigma-Aldrich
β-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, ≥250 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine muscle, Type X, ammonium sulfate suspension, ≥600 units/mg protein
Sigma-Aldrich
Lipase from wheat germ, Type I, lyophilized powder, 5-15 units/mg solid
Sigma-Aldrich
Pyruvate Kinase from rabbit muscle, Type II, ammonium sulfate suspension, 350-600 units/mg protein
Sigma-Aldrich
Lipase from porcine pancreas, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Sigma-Aldrich
β-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, ≥500 units/mg protein
Sigma-Aldrich
Lipase from porcine pancreas, Type VI-S, ≥20,000 units/mg protein, lyophilized powder
Sigma-Aldrich
Glycerokinase from Escherichia coli, lyophilized powder, 40-100 units/mg protein