生物反应器

上游 mAbs

开发和制造产品。

上游工作流程包括细胞收获的所有步骤，包括优化培养条件、细胞系的开发和。细胞系和产品表征, media development, 上游工艺化学品；以及将间歇、喂料间歇或灌注工艺放大到更大的生物反应器。上游生物制造的目标是提高细胞密度和产品滴度。

上游工艺

生物反应器

生物反应器有各种尺寸和规格（不锈钢、一次性使用或混合型），支持粘附和悬浮细胞培养。粘附细胞系可采用间歇或间歇喂养模式或培养基交换模式培养，而悬浮细胞可采用间歇、间歇喂养和灌流模式培养。无论采用哪种细胞类型或操作模式，生物反应器都必须能保证细胞的稳定生长、高细胞活力和稳健的蛋白质生产。在工艺开发过程中，缩小规模的小工作容积微生物反应器可用于预测大规模容器的性能，从而促进临床和商业生产或技术转让的放大过程。

我们围绕生物反应器提供的全面服务包括

Millipore^® Single-Use Biore/CN/zhactors With or Without Cell Retention System for Perfusion Oper/CN/zhations
Millipore^{® 预滤器}
Millipore^® 采样解决方案（自动在线系统，
Millipore® S/CN/zhampling Solutions（离线采样)
Millipore^® 过程分析技术（拉曼分析仪、自动无菌采样）
Millipore^® CTDMO、BioReli/CN/zhance® / Millipore^® 服务（细胞和病毒库测试、原料测试、批量采收释放测试/一次性使用和过滤器验证、设备安装、系统鉴定、系统培训、系统服务）
Millipore^® CTDMO/li>
Millipore^® Autom/CN/zhation /CN/zhand An/CN/zhalytics Softw/CN/zhare

相关资源

Data Sheet: Mobius Breez Microbioreactor
This document highlights the benefits of the Mobius^® Breez Microbioreactor, a 2 mL automated single-use perfusion cell culture platform.
Accelerate Perfusion Media Development and Predict Large-Scale Processes using a Single-use, Automated Microbioreactor
This page describes the use of the Mobius^® Breez Microbioreactor to rapidly screen and optimize media formulations for perfusion processes and to predict the performance of larger scale bioreactors as an alternative to traditional small-scale models.
Data Sheet: Mobius^® 3L Bioreactor
This document highlights the benefits of the Mobius^® 3L Bioreactor.
Data Sheet: Mobius^® iFlex Bioreactor
This document highlights the benefits of Mobius^® iFlex Bioreactor, a scalable family of next generation high-performance single-use modular bioreactors for fed-batch and perfusion processes.
Scalability and Performance of Mobius^® iFlex Bioreactors
Mobius^® iFlex Bioreactors are single-use, stirred tank systems designed to support traditional and intensified fed-batch as well as perfusion cell culture applications. Several performance attributes and scalability data for these bioreactors are described in this article.
Optimizing Upstream Media and Feeds in Bioprocessing
Developing a robust upstream cell culture process that maximizes productivity without increasing complexity relies on the right combination of bioprocess medium and feed and the ability to maintain tight control of culture conditions. Learn more how to achieve this.
Upstream Bioprocessing Feed Strategies and Scalability: A Case Study with Cellvento^® ModiFeed Prime COMP
Prior to scaling your upstream bioprocess to bioreactors, it’s best to set up small-scale cultures to determine the media and feed combinations as well as other parameters to best optimize growth, productivity, and product quality from your cells.
In-Line Real-Time Monitoring of CHO Cell Culture Process Parameters using Raman Spectroscopy
This article explains how ProCellics™ Raman Analyzer with Bio4C^® PAT Raman Software (the Raman PAT Platform) can be used to perform in-line and real-time measurement of TCD, VCD and mAb titer as well as the concentration of glucose, lactate, and ammonium in a bench-scale bioreactor, and how it compares with off line monitoring.
Brochure: Mission Control: Strategies for Effective Bioburden and Aseptic Control
In this series of articles, we provide information to highlight the differing objectives for bioburden control by unit operation and discuss the parameters that guide filter selection and optimization.
Cell Culture Media Filtration, Filter Selection and Sizing
The purpose of this application note is to provide estimated filtration areas for different sterilizing-grade filters with a panel of media used for Chinese Hamster Ovary (CHO) cell culture processes.
Cell Culture Media Mixing and Microfiltration: Reducing Risk with Single-use Technology
This case study presents our recommended methods for thoroughly mixing media, as well as reducing the risk of mycoplasma contamination through the use of 0.1 μm sterilizing-grade filters.