Volatile Fraction of Roasted Coffee Beans Using SPME on Omegawax™ 250
材料
SPME 光纤
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS)
needle size 24 ga, StableFlex, for use with autosampler分析色谱柱
标准
流动相组分
CONDITIONS
sample/matrix
2 g of coffee beans
SPME fiber
50/30 μm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U)
extraction
headspace, with a pre-equilibration period of 10 min. at 60 °C, and then a fiber exposure of 40 min. at 60 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm
desorption process
260 °C for 5 min
column
Omegawax 250, 30 m x 0.25 mm I.D., 0.25 μm (24136)
oven
40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280 °C (2 min)
inj. temp.
260 °C
detector
MS, m/z 40-400
carrier gas
helium, 34.8 cm/sec constant
injection
splitless
scan range
m/z 40-400
sample
Roasted coffee
liner
0.75 mm I.D. SPME/Splitless (2633901)
说明
分析说明
Headspace-SPME followed by GC analysis was used in this application to analyze volatile compounds in roasted coffee. SPME is ideal for analyzing the headspace of solid samples like coffee beans. The Carboxen/DVB/PDMS fiber is the fiber of choice for analyzing the wide range of analytes commonly found in roast coffee beans.The relatively polar Omegawax 250 GC column provided high resolution.
法律信息
Omegawax is a trademark of Sigma-Aldrich Co. LLC