UHPLC/MS/MS Analysis of 25 Small Molecule Pharmaceutical Compounds on Ascentis® Express RP-Amide
材料
分析色谱柱
标准
阿特拉津
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland阿米替林盐酸盐标准液 盐酸盐 溶液
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Haloperidol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®丙咪嗪标准液 CRM 盐酸盐 溶液
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®米赛林 盐酸盐 溶液
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®流动相组分
CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase
[A] 0.1% acetic acid, 5 mM ammonium acetate in water; [B] 5 mM ammonium acetate in acetonitrile
gradient
5% B for 1 min; to 43% B in 6 min; held for 1 min; to 80% B in 4 min; held for 1 min; to 5% B in 1 min; held for 3 min
flow rate
0.4 mL/min
detector
MS, ESI(+) MRM
injection
1 μL
说明
应用
This application highlights the advantage of an embedded polar group (EPG) HPLC phase for the separation of various small molecules. The use of an Ascentis® RP-Amide column allowed for the development of a single UPLC-MS/MS method for the analysis of 25 pharmaceuticals that varied significantly in both chemical and physical properties (log P, pKa, etc.). The presence of an embedded amide group allowed for retention of more polar analytes in comparison to a traditional C18 phase.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany