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A genome annotation-driven approach to cloning the human ORFeome.

A genome annotation-driven approach to cloning the human ORFeome.

Genome biology (2004-10-06)

John E Collins, Charmain L Wright, Carol A Edwards, Matthew P Davis, James A Grinham, Charlotte G Cole, Melanie E Goward, Begoña Aguado, Meera Mallya, Younes Mokrab, Elizabeth J Huckle, David M Beare, Ian Dunham

摘要

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

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脂肪酶来源于皱褶假丝酵母, Type VII, ≥700 unit/mg solid

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脂肪酶来源于猪胰腺, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

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脂肪酶丙烯酸树脂, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

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重组脂肪酶 B 南极假丝酵母来源于米曲霉, powder, beige, ~9 U/mg

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脂肪酶来源于猪胰腺, Type VI-S, ≥20,000 units/mg protein, lyophilized powder

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磷脂酶 A ₂ from honey bee venom (Apis mellifera), salt-free, lyophilized powder, 600-2400 units/mg protein

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脂肪酶来源于洋葱假单胞菌, powder, light beige, ≥30 U/mg

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脂肪酶来源于黑曲霉, powder (fine), ~200 U/g

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磷脂酶 A₂ 来源于猪胰腺, ammonium sulfate suspension, ≥600 units/mg protein

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脂肪酶来源于皱褶假丝酵母, lyophilized powder, ≥40,000 units/mg protein

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磷脂酶 A ₂ 来源于牛胰腺, lyophilized powder, ≥20 units/mg protein

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脂肪酶来源于小麦胚芽, Type I, lyophilized powder, 5-15 units/mg solid

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固定化脂肪酶来源于南极假丝酵母, beads, slightly brown, >2 U/mg

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脂肪酶来源于假单胞菌 属, Type XIII, lyophilized powder, ≥15 units/mg solid

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脂肪酶来源于米曲霉, lyophilized, powder, white, ~50 U/mg

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脂肪酶来源于米黑毛霉菌, powder, slightly brown, ~1 U/mg

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脂肪酶来源于皱褶假丝酵母, powder, yellow-brown, ≥2 U/mg

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Aconitase from porcine heart