Merck
CN
  • Molecular and biochemical characterisation of human short-chain dehydrogenase/reductase member 3 (DHRS3).

Molecular and biochemical characterisation of human short-chain dehydrogenase/reductase member 3 (DHRS3).

Chemico-biological interactions (2014-12-03)
Tereza Lundová, Lucie Zemanová, Beata Malčeková, Adam Skarka, Hana Štambergová, Jana Havránková, Miroslav Šafr, Vladimír Wsól
摘要

Dehydrogenase/reductase (SDR family) member 3 (DHRS3), also known as retinal short-chain dehydrogenase/reductase (retSDR1) is a member of SDR16C family. This family is thought to be NADP(H) dependent and to have multiple substrates; however, to date, only all-trans-retinal has been identified as a DHRS3 substrate. The reductive reaction catalysed by DHRS3 seems to be physiological, and recent studies proved the importance of DHRS3 for maintaining suitable retinoic acid levels during embryonic development in vivo. Although it seems that DHRS3 is an important protein, knowledge of the protein and its properties is quite limited, with the majority of information being more than 15 years old. This study aimed to generate a more comprehensive characterisation of the DHRS3 protein. Recombinant enzyme was prepared and demonstrated to be a microsomal, integral-membrane protein with the C-terminus oriented towards the cytosol, consistent with its preference of NADPH as a cofactor. It was determined that DHRS3 also participates in the metabolism of other endogenous compounds, such as androstenedione, estrone, and DL-glyceraldehyde, and in the biotransformation of xenobiotics (e.g., NNK and acetohexamide) in addition to all-trans-retinal. Purified and reconstituted enzyme was prepared for the first time and will be used for further studies. Expression of DHRS3 was shown at the level of both mRNA and protein in the human liver, testis and small intestine. This new information could open other areas of DHRS3 protein research.

材料
货号
品牌
产品描述

Sigma-Aldrich
十二烷基硫酸钠, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
Sigma-Aldrich
十二烷基硫酸钠, ACS reagent, ≥99.0%
Sigma-Aldrich
十二烷基硫酸钠, ReagentPlus®, ≥98.5% (GC)
Sigma-Aldrich
乙酸乙酯, anhydrous, 99.8%
Sigma-Aldrich
十二烷基硫酸钠, ≥99.0% (GC), dust-free pellets
Sigma-Aldrich
乙二胺四乙酸, ACS reagent, 99.4-100.6%, powder
Sigma-Aldrich
苯甲磺酰氟, ≥98.5% (GC)
Sigma-Aldrich
毛地黄皂苷, Used as non-ionic detergent
Sigma-Aldrich
十二烷基硫酸钠 溶液, BioUltra, for molecular biology, 10% in H2O
Sigma-Aldrich
磷酸钾, reagent grade, ≥98%
Sigma-Aldrich
乙二胺四乙酸, BioUltra, anhydrous, ≥99% (titration)
Sigma-Aldrich
磷酸钠, 96%
Sigma-Aldrich
乙二胺四乙酸 溶液, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
十二烷基硫酸钠 溶液, BioUltra, for molecular biology, 20% in H2O
Sigma-Aldrich
4-(2-氨乙基)苯磺酰氟 盐酸盐, ≥97.0% (HPLC)
Sigma-Aldrich
十二烷基硫酸钠, 92.5-100.5% based on total alkyl sulfate content basis
Sigma-Aldrich
乙二胺四乙酸, purified grade, ≥98.5%, powder
Sigma-Aldrich
十二烷基硫酸钠, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
十二烷基硫酸钠, BioXtra, ≥99.0% (GC)
Sigma-Aldrich
乙二胺四乙酸, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
苯甲磺酰氟, ≥99.0% (T)
Sigma-Aldrich
十二烷基硫酸钠, ≥90% ((Assay))
Sigma-Aldrich
1,2-二油酰--甘油基-3-磷酸胆碱, lyophilized powder
Sigma-Aldrich
毛地黄皂苷, ~50% (TLC)
Sigma-Aldrich
乙二胺四乙酸, 99.995% trace metals basis
Supelco
维生素A标准液 溶液, 100 μg/mL ± 25% (Refer to COA) (Ethanol with 0.1% (w/v) BHT), ampule of 1 mL, reference material, Cerilliant®
Supelco
十二烷基硫酸钠, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
乙酸乙酯, ≥99%, FCC, FG
Sigma-Aldrich
十二烷基硫酸钠, ≥98.0% (GC)
Sigma-Aldrich
乙酸乙酯, natural, ≥99%, FCC, FG