- Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis.
Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2014-08-05)
Peter Podolec, Alexandra Hengerics Szabó, Jaroslav Blaško, Róbert Kubinec, Renáta Górová, Jozef Višňovský, Anna Gnipová, Anton Horváth, Václav Bierhanzl, Tomáš Hložek, Radomír Čabala
PMID25089958
摘要
A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
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Supelco
N,O-双(三甲基硅)三氟乙酰胺(含三甲基氯硅烷), derivatization grade (GC derivatization), LiChropur™, contains 1% TMCS, 99% (excluding TMCS)
Supelco
N,O-双(三甲基硅)三氟乙酰胺(含三甲基氯硅烷), with 1% trimethylchlorosilane, derivatization grade (GC derivatization), LiChropur™
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甘油, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sigma-Aldrich
活性炭 Norit®, Norit® SX ultra, from peat, steam activated and acid washed, highly purified, powder