- Protease-activated alpha-2-macroglobulin can inhibit amyloid formation via two distinct mechanisms.
Protease-activated alpha-2-macroglobulin can inhibit amyloid formation via two distinct mechanisms.
FEBS letters (2013-01-29)
Amy R Wyatt, Patrick Constantinescu, Heath Ecroyd, Christopher M Dobson, Mark R Wilson, Janet R Kumita, Justin J Yerbury
PMID23353684
摘要
α(2)-Macroglobulin (α(2)M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of α(2)M with proteases results in an 'activated' conformation, where the proteases become covalently-linked within the interior of a cage-like structure formed by α(2)M. This study investigates, the effect of activation on the ability of α(2)M to inhibit amyloid formation by Aβ(1-42) and I59T human lysozyme and shows that protease-activated α(2)M can act via two distinct mechanisms: (i) by trapping proteases that remain able to degrade polypeptide chains and (ii) by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway.
材料
Product Number
品牌
产品描述
Sigma-Aldrich
胰蛋白酶 来源于猪胰腺, lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
胰蛋白酶 来源于牛胰腺, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Lysozyme 来源于鸡蛋白, BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein
Sigma-Aldrich
溶菌酶 人, Lysobac™, recombinant, expressed in rice, lyophilized powder, ≥100,000 units/mg protein
Sigma-Aldrich
胰蛋白酶 来源于牛胰腺, essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture
Sigma-Aldrich
胰蛋白酶 来源于牛胰腺, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
胰蛋白酶 来源于猪胰腺, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
TrypZean® 牛, recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
Sigma-Aldrich
溶菌酶 来源于人类中性粒细胞, ≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)